The international surgical journal with global reach

This is the Scientific Surgery Archive, which contains all randomized clinical trials in surgery that have been identified by searching the top 50 English language medical journal issues since January 1998. Compiled by Jonothan J. Earnshaw, former Editor-in-Chief, BJS

Oestrogen inhibits human colonic motility by a non‐genomic cell membrane receptor‐dependent mechanism. BJS 2009; 96: 817-822.

Published: 29th April 2009

Authors: A. M. Hogan, R. Kennelly, D. Collins, A. W. Baird, D. C. Winter

Background

Classical effects of oestrogen involve activation of target genes after binding nuclear receptors. Oestrogenic effects too rapid for DNA transcription (non‐genomic) are known to occur. The effect of oestrogen on colonic motility is unknown despite the prevalence of gastrointestinal symptoms in pregnant and premenopausal women.

Method

Histologically normal colon was obtained from proximal resection margins of colorectal carcinoma specimens. Circular smooth muscle strips were microdissected and suspended in organ baths under 1 g of tension. After equilibration, they were exposed to 17β‐oestradiol (n = 8) or bovine serum albumin (BSA)‐conjugated 17β‐oestradiol (n = 8). Fulvestrant, an oestrogen receptor antagonist, was added to some baths (n = 8). Other strips were exposed to calphostin C or cycloheximide. Carbachol was added in increasing concentrations and contractile activity was recorded isometrically.

Results

Oestrogen inhibited colonic contractility (mean difference 19·7 per cent; n = 8, P < 0·001). In keeping with non‐genomic, rapid‐onset steroid action, the effect was apparent within minutes and reversible. It was observed with both 17β‐oestradiol and BSA‐conjugated oestrogen, and was not altered by cycloheximide. Effects were inhibited by fulvestrant, suggesting receptor mediation.

Conclusion

Oestrogen decreases contractility in human colonic smooth muscle by a non‐genomic mechanism involving cell membrane coupling. Copyright © 2009 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.

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