This is the Scientific Surgery Archive, which contains all randomized clinical trials in surgery that have been identified by searching the top 50 English language medical journal issues since January 1998. Compiled by Jonothan J. Earnshaw, former Editor-in-Chief, BJS
Expression of cyclo‐oxygenase 1 and 2, prostaglandin E synthase and transforming growth factor β1, and their relationship with vascular endothelial growth factors A and C, in primary adenocarcinoma of the small intestine. BJS 2006; 93: 1424-1432.
Published: 4th October 2006
Authors: B. H. A. von Rahden, B. L. D. M. Brücher, C. Langner, J. R. Siewert, H. J. Stein, M. Sarbia et al.
Background
Primary adenocarcinomas of the small intestine are rare. The prostaglandin biosynthetic pathway plays a major role in carcinogenesis and is linked with angiogenesis in various tumours. Promotion of tumour growth by transforming growth factor (TGF) β may be mediated through the prostaglandin pathway.
Method
Expression of cyclo‐oxygenase (COX) 1 and 2, prostaglandin E synthase (PGES), TGF‐β1 and vascular endothelial growth factor (VEGF) A and C genes was analysed in 54 primary adenocarcinomas of the small intestine and corresponding normal intestinal mucosa. All patients had undergone surgical resection without previous antineoplastic therapy. Target gene expression was analysed at the mRNA level by reverse transcriptase–polymerase chain reaction and correlated with clinicopathological parameters as well as survival. COX‐2 protein expression was examined by immunohistochemistry.
Results
Expression of COX‐2 protein was detected immunohistochemically in 98 per cent of the carcinomas. COX‐1, COX‐2, VEGF‐A, VEGF‐C, PGES and TGF‐β1 mRNA expression varied markedly in different tumours, but all were overexpressed compared with levels in normal intestinal mucosa. There were significant associations between levels of COX‐1, COX‐2, TGF‐β1 and PGES mRNAs and those of VEGF‐A and VEGF‐C.
Conclusion
Correlations between levels of mRNA for COX‐1, COX‐2, TGF‐β1 and PGES and those for proangiogenic factors VEGF‐A and VEGF‐C suggest a role for these factors in the propagation of primary adenocarcinomas of the small intestine. Copyright © 2006 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.
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